Pheophorbides as photosensitizers for the photodynamic therapy of tumors

Charles Tanielian; Christian Wolff; Masami Kobayashi

doi:10.1117/12.199132

12 January 1995 Pheophorbides as photosensitizers for the photodynamic therapy of tumors

Charles Tanielian, Christian Wolff, Masami Kobayashi

Proceedings Volume 2325, Photodynamic Therapy of Cancer II; (1995) https://doi.org/10.1117/12.199132
Event: International Symposium on Biomedical Optics Europe '94, 1994, Lille, France

Abstract

Quantum yields for formation of singlet molecular oxygen have been measured for sodium pheophorbides (Na-Phdes) a and b in aqueous and non-aqueous media. Measurements have been made for both steady-state and pulsed laser excitation with the resultant singlet molecular oxygen being detected by photo-oxygenation reactions or time-resolved luminescence spectroscopy, respectively. Singlet oxygen production sensitized by Na-Phdes a or b is insignificant in aqueous media but occurs with a good efficiency in organic solvents. Plasmid DNA is efficiently photocleaved by Na-Phdes a and b in the absence of oxygen as well as in the presence of oxygen. Fluorescence microscopic observation shows a rapid incorporation of Na-Phde a into nuclei, mitochondria, and lysosome of human oral mucosa cells. In contrast Na-Phde b is incorporated only into the plasma membrane. The photodynamic activity of these pigments in living tissues is probably determined by the monomeric pigment molecules formed in hydrophobic cellular structures.

Citation Download Citation

Charles Tanielian, Christian Wolff, and Masami Kobayashi "Pheophorbides as photosensitizers for the photodynamic therapy of tumors", Proc. SPIE 2325, Photodynamic Therapy of Cancer II, (12 January 1995); https://doi.org/10.1117/12.199132

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