Urothelial conversion of 5-aminolevulinic acid to protoporphyrin IX following oral or intravesical administration

Ronald B. Moore; Gerald G. Miller; Kevin Brown; Rakesh Bhatnagar; John Tulip; Malcolm S. McPhee

doi:10.1117/12.203359

1 March 1995 Urothelial conversion of 5-aminolevulinic acid to protoporphyrin IX following oral or intravesical administration

Ronald B. Moore, Gerald G. Miller, Kevin Brown, Rakesh Bhatnagar, John Tulip, Malcolm S. McPhee

Author Affiliations +

Proceedings Volume 2371, 5th International Photodynamic Association Biennial Meeting; (1995) https://doi.org/10.1117/12.203359
Event: Fifth International Photodynamic Association Biennial Meeting, 1994, Amelia Island, FL, United States

Abstract

Preferential conversion of 5-aminolevulinic acid (5-ALA) to protoporphyrin-IX (Pp-IX) occurs in malignant tissue, with accumulation to diagnostic and therapeutic levels. Recent studies have suggested selective conversion in epithelial tissue following oral or intravenous administration. Topical application avoids systemic photosensitization. However, the glycosaminoglycan (GAG) layer lining the urinary bladder is believed to be a protective barrier generally limiting mucosal absorption. Our objective was to evaluate uptake and conversion of 5-ALA following intravesical or oral administration. Using a rat model, Pp-IX content within epithelial and muscularis layers was quantitated by fluorescence confocal microscopy. Following intravesical administration, Pp-IX accumulated predominantly in the urothelium; whereas following oral administration, Pp-IX accumulated in both the urothelium and muscularis. Intravesical 5-ALA administration is feasible and may afford selective photosensitization of the urothelium for treatment of carcinoma in situ.

Citation Download Citation

Ronald B. Moore, Gerald G. Miller, Kevin Brown, Rakesh Bhatnagar, John Tulip, and Malcolm S. McPhee "Urothelial conversion of 5-aminolevulinic acid to protoporphyrin IX following oral or intravesical administration", Proc. SPIE 2371, 5th International Photodynamic Association Biennial Meeting, (1 March 1995); https://doi.org/10.1117/12.203359

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