Immunosensing of 2,4-dichlorophenoxy acetic acid using biochemical amplification: influence of organic solvent on binding observed by evanescent field technology

Frank F. Bier; Walter F.M. Stocklein; Eva Ehrentreich-Forster; Frieder W. Scheller

doi:10.1117/12.224097

9 October 1995 Immunosensing of 2,4-dichlorophenoxy acetic acid using biochemical amplification: influence of organic solvent on binding observed by evanescent field technology

Frank F. Bier, Walter F.M. Stocklein, Eva Ehrentreich-Forster, Frieder W. Scheller

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Proceedings Volume 2504, Environmental Monitoring and Hazardous Waste Site Remediation; (1995) https://doi.org/10.1117/12.224097
Event: European Symposium on Optics for Environmental and Public Safety, 1995, Munich, Germany

Abstract

In conventional trace analysis pretreatment of a sample serves to enrich the substance to be detected. Some compounds polluting the environment are hydrophobic and stick to the soil matrix: therefore organic solvents often are used to prepare samples for environmental analysis. In biochemical analysis the enrichment step may be incorporated in the detection process. The most effective method is the use of immunochemistry; the matrix bound antibodies collect the analyte out of a highly diluted sample. Immunoassays therefore have been introduced successfully for environmental monitoring during the last decade; e.g., for pesticides many immunoassays have been developed and some of them are now commercially available; a recent review is given by Meulenberg et al. However, despite the fact that selectivity and sensitivity of immunoreactions is high, the capability of producing directly useful signals is poor. Many attempts have been made to overcome this limitation by the use of physical, chemical, or biochemical labels. But still there is need for more sensitivity than most immunoassays can achieve. On the other hand immunoassays usually are performed under physiological conditions and only rare data are available about antigen/antibody binding in non-aqueous systems.

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Frank F. Bier, Walter F.M. Stocklein, Eva Ehrentreich-Forster, and Frieder W. Scheller "Immunosensing of 2,4-dichlorophenoxy acetic acid using biochemical amplification: influence of organic solvent on binding observed by evanescent field technology", Proc. SPIE 2504, Environmental Monitoring and Hazardous Waste Site Remediation, (9 October 1995); https://doi.org/10.1117/12.224097

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