Superresolution in scanning fluorescence microscopy by means of image processing in the optical domain

U. Brand; J. Grochmalicki; G. Hester; I. Akduman; Edward Roy Pike M.D.

doi:10.1117/12.260799

6 December 1996 Superresolution in scanning fluorescence microscopy by means of image processing in the optical domain

U. Brand, J. Grochmalicki, G. Hester, I. Akduman, Edward Roy Pike M.D.

Proceedings Volume 2926, Optical Biopsies and Microscopic Techniques; (1996) https://doi.org/10.1117/12.260799
Event: BiOS Europe '96, 1996, Vienna, Austria

Abstract

We present a new technique in scanning fluorescence microscopy with high-NA lenses that offers improved resolution over confocal imaging modes by employing new optical masks. The pattern of this 2D element, which both reflects and transmits the light in the final image plane into subsequent detectors, is calculated by a singular value decomposition of the imaging operator. It brings about instant optical processing to extend the effective bandwidth of the transfer function clearly beyond that of the confocal microscope and leads to a resolution enhancement of up to 50%. We describe the development of the mask along with theoretical results.

Citation Download Citation

U. Brand, J. Grochmalicki, G. Hester, I. Akduman, and Edward Roy Pike M.D. "Superresolution in scanning fluorescence microscopy by means of image processing in the optical domain", Proc. SPIE 2926, Optical Biopsies and Microscopic Techniques, (6 December 1996); https://doi.org/10.1117/12.260799

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