Two-photon fluorescence of DNA-dye complexes

Lia A. Avanessian; Vladimir A. Hovanessian

doi:10.1117/12.273565

7 May 1997 Two-photon fluorescence of DNA-dye complexes

Lia A. Avanessian, Vladimir A. Hovanessian

Proceedings Volume 2980, Advances in Fluorescence Sensing Technology III; (1997) https://doi.org/10.1117/12.273565
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

Preliminary results of the investigation of two-photon excited fluorescence of acridine orange (AO) and ethidium bromide (EB) in complexes with DNA are presented. Spectrofluorometer based on picosecond Nd:YAG laser was used for investigations on two-photon (1064 nm, 1 mJ, 40 ps) and one-photon (532 and 355 nm) excitation. Highly purified DNA from beef spleen and AO, EB were used at the concentration of: AO- 15 (mu) M, EB- 12 (mu) M, DNA- 2-15 (mu) g/ml. The spectra of two-photon excited fluorescence of AO, EB and their complexes with DNA as well as kinetics of the intensification of dye's two-photon fluorescence during their interactions with DNA in dependence on DNA concentration were obtained. The intensity of fluorescence of AO and EB was increased maximum 2.4 and 8 times correspondingly. During the one-photon excitation corresponding values were for: AO-2.5 time and EB-10 time. The difference in the intensification of EB fluorescence connected with the difference of the change of one-photon and two-photon absorption coefficients during the interaction of the dye with DNA.

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Lia A. Avanessian and Vladimir A. Hovanessian "Two-photon fluorescence of DNA-dye complexes", Proc. SPIE 2980, Advances in Fluorescence Sensing Technology III, (7 May 1997); https://doi.org/10.1117/12.273565

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