All-solid-state TCPC instrument for dynamic lifetime measurements in sensitive DNA analysis

Rainer Erdmann; Michael Wahl; Kristian Lauritsen; Joerg Enderlein; Benjamin L. Legendre Jr.; Daryl C. Williams; Steven A. Soper

doi:10.1117/12.274364

22 May 1997 All-solid-state TCPC instrument for dynamic lifetime measurements in sensitive DNA analysis

Rainer Erdmann, Michael Wahl, Kristian Lauritsen, Joerg Enderlein, Benjamin L. Legendre Jr., Daryl C. Williams, Steven A. Soper

Author Affiliations +

Proceedings Volume 2985, Ultrasensitive Biochemical Diagnostics II; (1997) https://doi.org/10.1117/12.274364
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

We have constructed a compact, all solid state, time correlated single photon counting device for sensitive measurement of the time resolved behavior of DNA fragments passing through a capillary tube. The instrument consists of a pulsed diode laser PDL, a single photon avalanche diode SPCM-211 and a PC board containing all TCPC electronics. We achieved an overall instrument response function of the system of less than 300 ps. This is fast enough for measurement of fluorescence lifetimes. We demonstrated the utility and the sensitivity of the instrument; dynamic measurements of fluorescence decays for NIR dye-labelled nucleotide bases were measured during capillary electrophoresis. The results indicate that in a two-dye experiment the characteristic lifetime of the probe could be used to identify the terminal nucleotide base. The lifetimes of the tow dyes bound to DNA fragments were determined to be 670 ps and 530 ps.

Citation Download Citation

Rainer Erdmann, Michael Wahl, Kristian Lauritsen, Joerg Enderlein, Benjamin L. Legendre Jr., Daryl C. Williams, and Steven A. Soper "All-solid-state TCPC instrument for dynamic lifetime measurements in sensitive DNA analysis", Proc. SPIE 2985, Ultrasensitive Biochemical Diagnostics II, (22 May 1997); https://doi.org/10.1117/12.274364

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available