Analysis of human colon tissue using Raman spectroscopy

Sarah E. Cope; Tami L. Freeman; B. J. Rembacken; Mark R. Stringer; David N. Batchelder; Stanley B. Brown

doi:10.1117/12.297982

29 December 1997 Analysis of human colon tissue using Raman spectroscopy

Sarah E. Cope, Tami L. Freeman, B. J. Rembacken, Mark R. Stringer, David N. Batchelder, Stanley B. Brown

Proceedings Volume 3197, Optical Biopsies and Microscopic Techniques II; (1997) https://doi.org/10.1117/12.297982
Event: BiOS Europe '97, 1997, San Remo, Italy

Abstract

Near-infrared Raman microscopy was used to study normal, adenomatous and carcinomatous colon tissue in vitro. Samples were fixed in formalin and it was shown that this procedure did not alter the observed Raman peaks of the tissue, and is therefore a suitable method of fixation. The samples were observed visually to be of varying shades ranging from cream to dark brown; this was independent of whether the samples were cancerous or normal. Differences were seen between the Raman spectra of the different colored tissue with the spectra of brown tissue displaying a greater number of resolved peaks. We believe these differences may be due to differing amounts of blood present in the tissue. This must be considered when using Raman spectra for diagnostic work to ensure that differences ascribed to cancerous changes are not in fact due to differing amounts of blood within the sample.

Citation Download Citation

Sarah E. Cope, Tami L. Freeman, B. J. Rembacken, Mark R. Stringer, David N. Batchelder, and Stanley B. Brown "Analysis of human colon tissue using Raman spectroscopy", Proc. SPIE 3197, Optical Biopsies and Microscopic Techniques II, (29 December 1997); https://doi.org/10.1117/12.297982

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