Intracellular dynamics with the phase microscope Airyscan

Vladimir P. Tychinsky; Elena V. Perevedentseva; Tatiana V. Vyshenskaia; Georgy E. Kufal

doi:10.1117/12.297975

29 December 1997 Intracellular dynamics with the phase microscope Airyscan

Vladimir P. Tychinsky, Elena V. Perevedentseva, Tatiana V. Vyshenskaia, Georgy E. Kufal

Proceedings Volume 3197, Optical Biopsies and Microscopic Techniques II; (1997) https://doi.org/10.1117/12.297975
Event: BiOS Europe '97, 1997, San Remo, Italy

Abstract

Investigation of intracellular dynamics of Allium cepa inner epidermal cells are described. The applicability of the method for quantitative estimation of spatio-temporal phase fluctuations and the effect due to external factors is discussed. The analysis of time-sampled series allows one to detect the regions of various motility in cytoplasm. The intense Fourier-spectra harmonics in 0.2 - 8 Hz interval were observed inside a cell wall and cytoplasm. Regularly spaced 2- to 4-s long batches of 100-ms pulses at cell-wall sites are recorded. The phase-fluctuation intensity decreased and the frequencies of certain harmonics were shifted with lowering temperature. The advantages and specific features of the method are discussed.

Citation Download Citation

Vladimir P. Tychinsky, Elena V. Perevedentseva, Tatiana V. Vyshenskaia, and Georgy E. Kufal "Intracellular dynamics with the phase microscope Airyscan", Proc. SPIE 3197, Optical Biopsies and Microscopic Techniques II, (29 December 1997); https://doi.org/10.1117/12.297975

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