New method for measurement of in-vivo penetration of UVR into the crystalline lens

Per G. Soederberg; Stefan Loefgren; Ralph Michael; Xochitl Gonzales-Cirre

doi:10.1117/12.309434

1 June 1998 New method for measurement of in-vivo penetration of UVR into the crystalline lens

Per G. Soederberg, Stefan Loefgren, Ralph Michael, Xochitl Gonzales-Cirre

Proceedings Volume 3246, Ophthalmic Technologies VIII; (1998) https://doi.org/10.1117/12.309434
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

The penetration of 300 nm ultraviolet radiation (UVR) in the anterior cortex of the crystalline lens was 0.45 mm, penetration being expressed as the distance attenuating the transmittance to 1/e². The estimation is based on measurement of in vivo inactivation of lactate dehydrogenase (LDH) due to in vivo exposure to UVR 300 nm. The inactivation of LDH was independent of the latency interval after the exposure within 0 - 6 h. There was a 30 - 40% inactivation of LDH in the cornea that dropped to 0% in the inner anterior cortex. The currently described method allows spatially resolved measurement of the in vivo dose of UVR 300 nm within the cornea and the crystalline lens.

Citation Download Citation

Per G. Soederberg, Stefan Loefgren, Ralph Michael, and Xochitl Gonzales-Cirre "New method for measurement of in-vivo penetration of UVR into the crystalline lens", Proc. SPIE 3246, Ophthalmic Technologies VIII, (1 June 1998); https://doi.org/10.1117/12.309434

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