Biorecognition using gated ion channel biosensors: membranes supported by poly-1,2-diaminobenzene

Dorothea Anrather; Miriam Valina-Saba; Michaela M. Smetazko; Georg D. Bauer; Sigrid E. Schmutzer; Christof Weiss-Wichert; Thomas G. M. Schalkhammer

doi:10.1117/12.308017

1 May 1998 Biorecognition using gated ion channel biosensors: membranes supported by poly-1,2-diaminobenzene

Dorothea Anrather, Miriam Valina-Saba, Michaela M. Smetazko, Georg D. Bauer, Sigrid E. Schmutzer, Christof Weiss-Wichert, Thomas G. M. Schalkhammer

Author Affiliations +

Proceedings Volume 3253, Biomedical Sensing and Imaging Technologies; (1998) https://doi.org/10.1117/12.308017
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

The device presented is based on a supported membrane incorporating modified ion channels. Various analytes induced down regulation of the ion flux by interaction with specific binding sites or artificial ligands attached near the channel entrance. To set up such a device new types of stabilizing membrane supports had to be developed. The new support presented was deposited by electyropolymerization of 1,2-diaminobenzene onto metal electrodes thus exhibiting a highly charged surface. Negatively charged lipids formed SA- membranes tightly bound to that gel surface. Mixtures of biological lipids and archaeabacteria type bolaamphiphilic lipids minimized lipids minimized floating of the membrane layer. Various ligands were bound to the modified Bisgramicidin-A to interact with their specific antibodies. It turned out that it is vital to accurately coupled the ligand at a single functional hydroxy-group. Sensors were optimized using a metal/ligand and 2,4- dinitrophenol/polyclonal antibody setup. Summing up, highly stable supported membranes were formed, ion channel were functionally integrated into these membranes and molecule interactions of analytes with embedded ion channels were monitored.

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Dorothea Anrather, Miriam Valina-Saba, Michaela M. Smetazko, Georg D. Bauer, Sigrid E. Schmutzer, Christof Weiss-Wichert, and Thomas G. M. Schalkhammer "Biorecognition using gated ion channel biosensors: membranes supported by poly-1,2-diaminobenzene", Proc. SPIE 3253, Biomedical Sensing and Imaging Technologies, (1 May 1998); https://doi.org/10.1117/12.308017

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