Angular dependence of light scattering by biological tissue

Rene Alexander Bolt; Johannes Sake Kanger; Frits F. M. de Mul

doi:10.1117/12.336932

15 January 1999 Angular dependence of light scattering by biological tissue

Rene Alexander Bolt, Johannes Sake Kanger, Frits F. M. de Mul

Proceedings Volume 3570, Biomedical Sensors, Fibers, and Optical Delivery Systems; (1999) https://doi.org/10.1117/12.336932
Event: BiOS Europe '98, 1998, Stockholm, Sweden

Abstract

We study the influence of analytes on the scattering phase function of tissue mimicking phantom materials. These analytes change the scattering of the sample by a small fraction. We developed a system for measuring the scattering phase function of turbid media and used it to study the influence of analytes on the scattering phase function and the scattering cross section. Measurement of the scattering phase function of complex turbid media, such as human tissue mimicking phantoms, provides a means of increasing the accuracy of calculations of light scattering by such media. Instead of an assumed - generally simplified - phase function, one can use a more realistic phase function in e.g., Monte Carlo simulations. It also provides information on the scattering and absorption properties of the medium. The influence of analyte concentration is derived from changes in scattering, making it a relative measurement. This has the advantage that there is no need to determine the scattering itself. This paper discusses the experimental set-up and test measurements on monodisperse polystyrene latex suspension in comparison to Monte Carlo simulations, the possibility of extracting the scattering phase function. We also present measurements of the influence of an analyte on the scattering properties of biological tissue in vitro. The change in scattering coefficient found from these measurements is in good agreement with theoretically predicted values.

Citation Download Citation

Rene Alexander Bolt, Johannes Sake Kanger, and Frits F. M. de Mul "Angular dependence of light scattering by biological tissue", Proc. SPIE 3570, Biomedical Sensors, Fibers, and Optical Delivery Systems, (15 January 1999); https://doi.org/10.1117/12.336932

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