Ellipsometric studies on deposition of complement from human serum to IgG and IgA immobilized on hydrophobic silicon

Pentti Tengvall; Agneta Askendal; Ingemar Lundstroem

doi:10.1117/12.336919

15 January 1999 Ellipsometric studies on deposition of complement from human serum to IgG and IgA immobilized on hydrophobic silicon

Pentti Tengvall, Agneta Askendal, Ingemar Lundstroem

Author Affiliations +

Proceedings Volume 3570, Biomedical Sensors, Fibers, and Optical Delivery Systems; (1999) https://doi.org/10.1117/12.336919
Event: BiOS Europe '98, 1998, Stockholm, Sweden

Abstract

Human immunoglobulin G (IgG) and colostrum immunoglobulin A (IgA) were absorbed to hydrophobic silicon and the deposition of complement (C) was studied by ellipsometry- antibody techniques after incubation at 37 degree(s)C in human serum for up to 1 hour. In parallel experiments soluble iC3b, C4d and Bb were detected by ELISA-techniques. IgG coated surfaces rapidly activated the classical pathway and caused deposition of anti-C3c, antiproperdin, and during short serum incubation times also of anti-C1q and anti-IgG. The IgA-coated surfaces activated the alternative pathway and displayed a lag phase in the complement protein deposition. Anti-IgG, Clq, -C4, -factor H and -factor B were not deposited into IgA-surfaces at any time. Ellipsometry and antibody techniques offer a convenient and rapid means to display activation of the complement system by solid light-reflecting surfaces and facilitate a time-resolved determination of the activation pathway(s).

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Pentti Tengvall, Agneta Askendal, and Ingemar Lundstroem "Ellipsometric studies on deposition of complement from human serum to IgG and IgA immobilized on hydrophobic silicon", Proc. SPIE 3570, Biomedical Sensors, Fibers, and Optical Delivery Systems, (15 January 1999); https://doi.org/10.1117/12.336919

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KEYWORDS

Proteins

Silicon

Optical spheres

Ellipsometry

Natural surfaces

Bioalcohols

Glasses

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