Confocal imaging of biological tissues using second harmonic generation

Beop-Min Kim; Patrick Christian Stoller; Karen M. Reiser; Juergen P. Eichler; Ming Yan; Alexander M. Rubenchik; Luiz Barroca Da Silva

doi:10.1117/12.388066

13 June 2000 Confocal imaging of biological tissues using second harmonic generation

Beop-Min Kim, Patrick Christian Stoller, Karen M. Reiser, Juergen P. Eichler, Ming Yan, Alexander M. Rubenchik, Luiz Barroca Da Silva

Author Affiliations +

Proceedings Volume 3914, Laser-Tissue Interaction XI: Photochemical, Photothermal, and Photomechanical; (2000) https://doi.org/10.1117/12.388066
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

A confocal microscopy imaging system was devised to selectively detect second harmonic signals generated by biological tissues. Several types of biological tissues were examined using this imaging system, including human teeth, bovine blood vessels, and chicken skin. All these tissues generated strong second harmonic signals. There is considerable evidence that the source of these signals in tissue is collagen. Collagen, the predominant component of most tissues, is known to have second order nonlinear susceptibility. This technique may have diagnostic usefulness in pathophysiological conditions characterized by changes in collagen structure including malignant transformation of nevi, progression of diabetic complications, and abnormalities in wound healing.

Citation Download Citation

Beop-Min Kim, Patrick Christian Stoller, Karen M. Reiser, Juergen P. Eichler, Ming Yan, Alexander M. Rubenchik, and Luiz Barroca Da Silva "Confocal imaging of biological tissues using second harmonic generation", Proc. SPIE 3914, Laser-Tissue Interaction XI: Photochemical, Photothermal, and Photomechanical, (13 June 2000); https://doi.org/10.1117/12.388066

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