Time-domain 2D fluorescence lifetime microscopic measurement

Jun Le Qu; Hanben Niu; Ji Li; Xiaomei Kuo; Junlan Zhou

doi:10.1117/12.384525

25 April 2000 Time-domain 2D fluorescence lifetime microscopic measurement

Jun Le Qu, Hanben Niu, Ji Li, Xiaomei Kuo, Junlan Zhou

Proceedings Volume 3977, Medical Imaging 2000: Physics of Medical Imaging; (2000) https://doi.org/10.1117/12.384525
Event: Medical Imaging 2000, 2000, San Diego, CA, United States

Abstract

In this paper a new technique of time-domain 2-D fluorescence lifetime microscopic measurement is presented. A fluorescence lifetime imaging microscope system based on a synchroscan streak camera is developed, and a data processing method of this technique is described. Reference sample is used to test the system. Experimental results show that, when magnification of the microscope is 100X, temporal resolution of the system is 2ps and spatial resolution is 8 micrometer. When a finer aperture mask and a micro-shift-mechanical system are used, spatial resolution of less than 1 micrometer can be obtained.

Citation Download Citation

Jun Le Qu, Hanben Niu, Ji Li, Xiaomei Kuo, and Junlan Zhou "Time-domain 2D fluorescence lifetime microscopic measurement", Proc. SPIE 3977, Medical Imaging 2000: Physics of Medical Imaging, (25 April 2000); https://doi.org/10.1117/12.384525

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