Physical perturbation for fluorescent characterization of microorganism particles

Burt V. Bronk; Azadeh Shoaibi; Raphael Nudelman; Agnes N. Akinyemi

doi:10.1117/12.394077

28 July 2000 Physical perturbation for fluorescent characterization of microorganism particles

Burt V. Bronk, Azadeh Shoaibi, Raphael Nudelman, Agnes N. Akinyemi

Proceedings Volume 4036, Chemical and Biological Sensing; (2000) https://doi.org/10.1117/12.394077
Event: AeroSense 2000, 2000, Orlando, FL, United States

Abstract

The motivation for using response to physical perturbation to classify microparticles came from our previous experiments with Dipicolinic Acid (DPA). DPA as a calcium complex is a major component of bacterial spores, constituting more than 5% of their dry weight. It is not commonly found in other natural products and therefore its presence is indicative of the presence of bacterial spores. Previous schemes utilizing the presence of DPA to detect these spores have relied on fluorescence which occurs when lanthanide metals (e.g., terbium) are added to a solution where the presence of DPA is to be determined. We have recently demonstrated that changes in the fluorescence of DPA can be stimulated without the addition of such reagents. Thus after exposure to UV light, a substantial increase of fluorescence emitted by DPA solutions with a peak at 410 nm occurs for excitation light with wavelength less than approximately 305 nm.

Citation Download Citation

Burt V. Bronk, Azadeh Shoaibi, Raphael Nudelman, and Agnes N. Akinyemi "Physical perturbation for fluorescent characterization of microorganism particles", Proc. SPIE 4036, Chemical and Biological Sensing, (28 July 2000); https://doi.org/10.1117/12.394077

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