Early responses of human cancer cells upon photodynamic treatment monitored by laser phase microscopy

Theo A. Roelofs; Georgi Graschew; Elena V. Perevedentseva; Stefan Rakowsky; Cathrin Dressler; Juergen Beuthan; Peter M. Schlag

doi:10.1117/12.424449

9 April 2001 Early responses of human cancer cells upon photodynamic treatment monitored by laser phase microscopy

Theo A. Roelofs, Georgi Graschew, Elena V. Perevedentseva, Stefan Rakowsky, Cathrin Dressler, Juergen Beuthan, Peter M. Schlag

Author Affiliations +

Proceedings Volume 4248, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy X; (2001) https://doi.org/10.1117/12.424449
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

Photodynamic treatment of cancer cells is known to eventually cause cell death in most cases. The precise pathways and the time course seem to vary among different cell types and modes of photodynamic treatment. In this contribution, the focus was put on the responses of human colon carcinoma cells HCT-116 within the first 15 minutes after laser irradiation in the presence of Photofrin® II (PII). To monitor the cell response in this early time period laser phase microscopic imaging was used, a method sensitive to changes in overall cell shape and intracellular structures, mediated by changes in the local refractive index. Laser irradiation of cells loaded with PII induced a significant reduction of the phase shifts, which probably reflects the induced damage to the different cellular membrane structures. The data suggest that even within the first 30 s after the onset of laser illumination, a significant reduction of the phase shifts can be detected. These results underline that laser phase microscopy is a suitable diagnostic tool for cellular research, also in the early time domain.

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Theo A. Roelofs, Georgi Graschew, Elena V. Perevedentseva, Stefan Rakowsky, Cathrin Dressler, Juergen Beuthan, and Peter M. Schlag "Early responses of human cancer cells upon photodynamic treatment monitored by laser phase microscopy", Proc. SPIE 4248, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy X, (9 April 2001); https://doi.org/10.1117/12.424449

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KEYWORDS

Phase shifts

Microscopy

Laser therapeutics

Image acquisition

Laser irradiation

Data acquisition

Confocal microscopy

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