Effects of LLLT on malignant cells: study in vitro

Antonio Luiz Barbosa Pinheiro; Silene Carneiro Nascimento; Alessandro L. B. Vieira; Aluizio Barros Rolim; Pedro Soriano da Silva; Aldo Brugnera Jr.; Fatima A. A. Zanin D.D.S.

doi:10.1117/12.424490

27 April 2001 Effects of LLLT on malignant cells: study in vitro

Antonio Luiz Barbosa Pinheiro, Silene Carneiro Nascimento, Alessandro L. B. Vieira, Aluizio Barros Rolim, Pedro Soriano da Silva, Aldo Brugnera Jr., Fatima A. A. Zanin D.D.S.

Author Affiliations +

Proceedings Volume 4249, Lasers in Dentistry VII; (2001) https://doi.org/10.1117/12.424490
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

In addition to our previous report on the effects of LLLT on the proliferation of laryngeal carcinoma cells in which it was found that irradiation H.Ep.2 cells with 670nm laser results in increased cell proliferation, ti was decided to evaluate the effect of increased does of laser light. The aim of this study was to assess the effect off 635 and 670 nm laser irradiation of H.Ep.2 cells in vitro using MTT. The cells, obtained from SCC of the larynx, were routinely processed from defrost to the experimental condition. The cultures were kept either at 5 or 10 percent of FBS. Twenty- four hours after transplantation, the cells were irradiated with laser light at local light doses between 0.04 and 4.8 X 10⁴ Jm^-2. For 670 nm, significant differences in the proliferation were observed between the two concentrations of FBS and between irradiated cultures and controls. Although the results were not significant, 635 nm irradiated cells also proliferated more than non- irradiated ones. This occurred under both conditions of nutrition. It is concluded, that irradiation with 670 nm laser light applied at doses between 0.04 and 4.8 X 10⁴ Jm^-2 could significantly increase proliferation of laryngeal cancer cells.

Citation Download Citation

Antonio Luiz Barbosa Pinheiro, Silene Carneiro Nascimento, Alessandro L. B. Vieira, Aluizio Barros Rolim, Pedro Soriano da Silva, Aldo Brugnera Jr., and Fatima A. A. Zanin D.D.S. "Effects of LLLT on malignant cells: study in vitro", Proc. SPIE 4249, Lasers in Dentistry VII, (27 April 2001); https://doi.org/10.1117/12.424490

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available