Depth penetration limit to optical coherence microscopy in turbid media: the effect of multiply scattered light detection on image contrast and resolution

Kostadinka K. Bizheva; David A. Boas

doi:10.1117/12.427871

23 May 2001 Depth penetration limit to optical coherence microscopy in turbid media: the effect of multiply scattered light detection on image contrast and resolution

Kostadinka K. Bizheva, David A. Boas

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Proceedings Volume 4251, Coherence Domain Optical Methods in Biomedical Science and Clinical Applications V; (2001) https://doi.org/10.1117/12.427871
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

It is a well established fact that detection of multiply scattered light causes image quality degradation in optical microscopy. In this study experimental and computational methods were employed to examine the effect of multiply scattered light detection on the image contrast, resolution and image penetration depth of optical coherence microscopy (OCM) in turbid media. Dynamic low coherence interferometry (DLCI), a method sensitive to changes in the photon momentum transfer resulting from scattering, was used to study the OCM background light rejection ability in homogeneous turbid media and to determine the optical depths at which single scattered, multiply scattered and diffuse light constitute the dominant components of the measured intensity. OCM resolution measurements performed at various optical depths in scattering media revealed correlation between loss of OCM spatial resolution and spectrum linewidth broadening (DLCI) resulting from detection of multiply scattered light. The data from the resolution measurements was used to determine the dependence of image contrast, resolution and penetration depth on the optical properties of the turbid background and the instrument imaging geometry. In addition, a Monte Carlo model was designed to examine the contribution of background light (light that does not carry any information about the imaged object and multiply scattered while traversing the turbid layer above) to loss of OCM image quality. The results from this study contribute to our understanding of the practical limits to OCM imaging in turbid media.

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Kostadinka K. Bizheva and David A. Boas "Depth penetration limit to optical coherence microscopy in turbid media: the effect of multiply scattered light detection on image contrast and resolution", Proc. SPIE 4251, Coherence Domain Optical Methods in Biomedical Science and Clinical Applications V, (23 May 2001); https://doi.org/10.1117/12.427871

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KEYWORDS

Light scattering

Image resolution

Scattering

Image quality

Monte Carlo methods

Anisotropy

Optical properties

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