Time-resolved fluorescence spectroscopy of dopamine in single cells

Michiaki Mabuchi; Junichi Shimada; Koichi Okamoto; Yoichi Kawakami; Shigeo Fujita; Kazumi Matsushige

doi:10.1117/12.426738

10 May 2001 Time-resolved fluorescence spectroscopy of dopamine in single cells

Michiaki Mabuchi, Junichi Shimada, Koichi Okamoto, Yoichi Kawakami, Shigeo Fujita, Kazumi Matsushige

Proceedings Volume 4252, Advances in Fluorescence Sensing Technology V; (2001) https://doi.org/10.1117/12.426738
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

Dopamine hydrochloric acid salt in aqueous solution was excited at 266 nm Al₂O₃:Ti laser and the sufficient fluorescence emission peaking at 330 nm was detected with a streak camera. The fluorescence decay curve was fitted by 1- exponential functions, with the lifetime of approximately 0.80 ns. The influence of deep-UV laser excitation on cells is also discussed for the direct observation of dopamine in the living cells. In addition, it is needed to detect the dopamine fluorescence in the living cell sensitively, and separately from emission of other fluorescent species. When instrumental arrangement and time-resolved spectral analysis can make it possible to solve such problems, direct visualization of the secretion process of individual cells will be achieved by the laser-induced native fluorescence imaging microscopy, without using any additional fluorescent probes. This quantitative imaging technique will provide a useful noninvasive approach for the study of dynamic cellular changes and the understanding of the molecular mechanisms of information transporting processes.

Citation Download Citation

Michiaki Mabuchi, Junichi Shimada, Koichi Okamoto, Yoichi Kawakami, Shigeo Fujita, and Kazumi Matsushige "Time-resolved fluorescence spectroscopy of dopamine in single cells", Proc. SPIE 4252, Advances in Fluorescence Sensing Technology V, (10 May 2001); https://doi.org/10.1117/12.426738

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