DNA biochips based on surface-enhanced fluorescence (SEF) for high-throughput interaction studies

Norbert Stich; Christian Mayer; Georg D. Bauer; Thomas G. M. Schalkhammer

doi:10.1117/12.446669

24 October 2001 DNA biochips based on surface-enhanced fluorescence (SEF) for high-throughput interaction studies

Norbert Stich, Christian Mayer, Georg D. Bauer, Thomas G. M. Schalkhammer

Proceedings Volume 4434, Hybrid and Novel Imaging and New Optical Instrumentation for Biomedical Applications; (2001) https://doi.org/10.1117/12.446669
Event: European Conferences on Biomedical Optics, 2001, Munich, Germany

Abstract

Molecular interaction especially biorecognitive binding can be visualized by metal cluster enhanced fluorescence. Fluorescent molecules that are bound within the electromagnetic field of a layer of metal clusters exhibit a strong boost in excitation as well as emission. We present a study, using novel surface enhanced chips in glass-slide-format, their set up, the micro arraying onto the surface, and the hybridization of oligonucleotides on these chips. Compared to standard (glass slide) DNA chips, performance, fluorescent signals as well as signal to noise ratio were considerably higher.

Citation Download Citation

Norbert Stich, Christian Mayer, Georg D. Bauer, and Thomas G. M. Schalkhammer "DNA biochips based on surface-enhanced fluorescence (SEF) for high-throughput interaction studies", Proc. SPIE 4434, Hybrid and Novel Imaging and New Optical Instrumentation for Biomedical Applications, (24 October 2001); https://doi.org/10.1117/12.446669

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