Excitation wavelength tunable Raman spectroscopy: study of preresonance enhancement of hemoglobin with 700-860 nm excitation wavelengths

Hidetoshi Sato; Akifumi Ikehata; Satoshi Wada; Hideo Tashiro

doi:10.1117/12.450164

27 November 2001 Excitation wavelength tunable Raman spectroscopy: study of preresonance enhancement of hemoglobin with 700-860 nm excitation wavelengths

Hidetoshi Sato, Akifumi Ikehata, Satoshi Wada, Hideo Tashiro

Author Affiliations +

Proceedings Volume 4491, Subsurface and Surface Sensing Technologies and Applications III; (2001) https://doi.org/10.1117/12.450164
Event: International Symposium on Optical Science and Technology, 2001, San Diego, CA, United States

Abstract

We have developed an excitation wavelength tunable Raman system which consists of a spectrometer, a CCD detector and an electronically tuned Ti:sapphire (ETT) laser. All those components are controlled with a single computer. This system is useful for Raman excitation profile measurements. A new technique was developed to calibrate deviation of wavelengths of ETT laser from that of Raman spectrometer within +/- 1.5cm^-1 of error. Obtained Raman spectra of hemoglobin measured with various excitation wavelengths in 700-860 nm range showed characteristic change of their intensities in some of Raman bands. Assignments of Raman bands were carried out sufficiently in help with change in intensity of the bands which reflect symmetric character of the responsible vibrational modes with the change in excitation wavelength.

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Hidetoshi Sato, Akifumi Ikehata, Satoshi Wada, and Hideo Tashiro "Excitation wavelength tunable Raman spectroscopy: study of preresonance enhancement of hemoglobin with 700-860 nm excitation wavelengths", Proc. SPIE 4491, Subsurface and Surface Sensing Technologies and Applications III, (27 November 2001); https://doi.org/10.1117/12.450164

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