What are the targets of photodynamic therapy?

David Kessel; Michelle Castelli; John Reiners Jr.

doi:10.1117/12.469340

6 June 2002 What are the targets of photodynamic therapy?

David Kessel, Michelle Castelli, John Reiners Jr.

Proceedings Volume 4612, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XI; (2002) https://doi.org/10.1117/12.469340
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

We previously classified photosensitizing agents based on sites of photodamage: mitochondria, lysosomes and the plasma membrane. More recent studies have indicated that the first target for the mitochondrial sensitizers is the anti-apoptotic protein bcl-2. Loss of bcl-2 results in a high bax/bcl-2 ratio, leading to an apoptotic response involving cytochrome c translocation. PDT with sensitizers that target lysosomes causes the release of lysosomal proteases that catalyze cleavage of the protein bid to a truncated form that can also initiate an apoptotic response. When the PDT target includes the plasma membrane, we found a greatly impaired apoptotic response, associated with caspase-3 photodamage. Sensitizers that localize in only lysosomes also catalyze variable levels of caspase photodamage, but apoptosis is only slightly impaired. PDT appears to be a highly-selective means for eradication of bcl-2, lysosomes or caspases, and can be a potentially useful tool in apoptosis research.

Citation Download Citation

David Kessel, Michelle Castelli, and John Reiners Jr. "What are the targets of photodynamic therapy?", Proc. SPIE 4612, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XI, (6 June 2002); https://doi.org/10.1117/12.469340

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