Fluorescence in the fiber optic HPTLC-method

Karl-Friedrich Klein; Bernd Spangenberg; Bjoern Arenz

doi:10.1117/12.463816

26 March 2002 Fluorescence in the fiber optic HPTLC-method

Karl-Friedrich Klein, Bernd Spangenberg, Bjoern Arenz

Proceedings Volume 4616, Optical Fibers and Sensors for Medical Applications II; (2002) https://doi.org/10.1117/12.463816
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

The sample analysis with Thin Layer Chromatography is an off-line method, in contrast to other separation methods. In addition to the sample preparation, the key-step is the in- situ densitometry for qualitative or quantitative purposes by measuring the optical density of the separated spots directly on the plate. With a newly developed HPTLC-system using fiber-optic and diode-array detection, simultaneous measurements at different wavelengths can be performed. The fiber-optic assembly consists of UV-improved silica-based fibers transporting the light and illuminating the surface of the TLC-plate. The light is scattered and reflected from the surface and finally collected by the reading fibers, carrying the relevant information. Up to now, only the absorption mode was used. However, using fluorescent substances, the light-power in a well-defined wavelength- region can be increased: the collected light from the illuminated spot of a substance on the TLC-plate is higher than the reference probe without substances. Fluorescent measurements are possible without filters or special lamps and the improvement of signal-to-noise ratios is significant. Due to the detailed structure of the PAH's absorption- and fluorescence spectra, diode-array HPTLC makes quantification of 16 PAH, recommended for monitoring by EPA, on one track possible. There is an additional advantage: although the spatial resolution of the TLC-separation of PAH is not sufficient for in situ quantitative densitometric analysis each of the 16 compounds can be quantified with the new method using suitable wavelengths.

Citation Download Citation

Karl-Friedrich Klein, Bernd Spangenberg, and Bjoern Arenz "Fluorescence in the fiber optic HPTLC-method", Proc. SPIE 4616, Optical Fibers and Sensors for Medical Applications II, (26 March 2002); https://doi.org/10.1117/12.463816

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available