Microarray platform for the creation of a matrix of site-specific transformed cells

Endre Szili; Helmut Thissen; Jason P. Hayes; Joe G. Shapter; Nicolas Voelcker

doi:10.1117/12.468658

14 November 2002 A microarray platform for the creation of a matrix of site-specific transformed cells

Endre Szili, Helmut Thissen, Jason P. Hayes, Joe G. Shapter, Nicolas Voelcker

Proceedings Volume 4937, Biomedical Applications of Micro- and Nanoengineering; (2002) https://doi.org/10.1117/12.468658
Event: SPIE's International Symposium on Smart Materials, Nano-, and Micro- Smart Systems, 2002, Melbourne, Australia

Abstract

This paper investigates the ability to modify the surface of silicon wafers for selective cell adhesion and the efficacy of solid phase transfections on the modified surface. Silicon surfaces are first modified by plasma polymerization of allylamine (ALAPP) and subsequent grafting of a protein-resistant layer of poly(ethylene oxide) (PEO) on the plasma polymer surface. Spatially controlled excimer ablation was then used to pattern the graft-copolymer surface for selective cell adhesion. X-ray photoelectron spectroscopy and contact angle measurements confirmed the creation of 2D patterns with different surface chemistry. Cell culture experiments with HEK 293 cells showed that cell attachment is limited to the ablated areas. Furthermore, cells could be transformed with plasmid DNA containing the gene for green fluorescent protein. Therefore, the biochip platform described in this paper, has the potential to be developed into a high-density array for analyzing gene products produced from a matrix of living cells.

Citation Download Citation

Endre Szili, Helmut Thissen, Jason P. Hayes, Joe G. Shapter, and Nicolas Voelcker "A microarray platform for the creation of a matrix of site-specific transformed cells", Proc. SPIE 4937, Biomedical Applications of Micro- and Nanoengineering, (14 November 2002); https://doi.org/10.1117/12.468658

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