Assessing changes in physical lens dimensions during immersion in tissue preservation media using shadowphotogrammetry

Alexander Rosen; David B. Denham; Fabrice Manns; Viviana Fernandez; Joseph Stoiber M.D.; Robert Augusteyn; Jean-Marie A. Parel

doi:10.1117/12.482374

14 July 2003 Assessing changes in physical lens dimensions during immersion in tissue preservation media using shadowphotogrammetry

Alexander Rosen, David B. Denham, Fabrice Manns, Viviana Fernandez, Joseph Stoiber M.D., Robert Augusteyn, Jean-Marie A. Parel

Author Affiliations +

Proceedings Volume 4951, Ophthalmic Technologies XIII; (2003) https://doi.org/10.1117/12.482374
Event: Biomedical Optics, 2003, San Jose, CA, United States

Abstract

Purpose: To develop a technique to assess isotonic solutions for crystalline lens preservation. Methods: BSS, Ringer’s, Dulbecco’s Modified Eagle Medium (DMEM), and TC 199 have been selected for experimentation. Donor human cadaver lenses are extracted by cutting the zonules and vitreous adherences. A custom-made testing cell and modified optical comparator are used for imaging, and the lens diameter and thickness profiles are measured using digital photography and a graphics program. Measurements are taken every 5 minutes for the 1st hour and then every 15 minutes for 4 hrs. The volume of the lens at each interval is approximated, assuming rotational symmetry, by using the equation for the volume of an ellipsoid. The changes in diameter, thickness, and volume versus time are analyzed to compare the effect of each solution. Results: The measurement resolution for the digital technique is 13μm. Lens thickness changed more significantly than diameter and volume. All four solutions produced similar preliminary results with maximum diameter, thickness, and volume changes of approximately -2%, +6%, and +3%, respectively. Conclusion: Shadowphotogrammetry can measure the lens physical dimensions to +/-13um and can be used to determine the effect of preservation media. This technique may be useful for assessing the physical effects of chemical and biological substances on the lens osmotic transport system.

Citation Download Citation

Alexander Rosen, David B. Denham, Fabrice Manns, Viviana Fernandez, Joseph Stoiber M.D., Robert Augusteyn, and Jean-Marie A. Parel "Assessing changes in physical lens dimensions during immersion in tissue preservation media using shadowphotogrammetry", Proc. SPIE 4951, Ophthalmic Technologies XIII, (14 July 2003); https://doi.org/10.1117/12.482374

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available