Multiphoton imaging: a view to understanding sulfur mustard lesions

Robert J. S. Werrlein; Janna S. Madren-Whalley

doi:10.1117/12.478035

10 July 2003 Multiphoton imaging: a view to understanding sulfur mustard lesions

Robert J. S. Werrlein, Janna S. Madren-Whalley

Proceedings Volume 4963, Multiphoton Microscopy in the Biomedical Sciences III; (2003) https://doi.org/10.1117/12.478035
Event: Biomedical Optics, 2003, San Jose, CA, United States

Abstract

It is well known that topical exposure to sulfur mustard (SM) produces persistent, incapacitating blisters of the skin. However, the primary lesions effecting epidermal-dermal separation and disabling of mechanisms for cutaneous repair remain uncertain. Immunofluorescent staining plus multiphoton imaging of human epidermal tissues and keratinocytes exposed to SM (400 μM x 5 min)have revealed that SM disrupts adhesion-complex molecules which are also disrupted by epidermolysis bullosa-type blistering diseases of the skin. Images of keratin-14 showed early, progressive, postexposure collapse of the K5/K14 cytoskeleton that resulted in ventral displacement of the nuclei beneath its collapsing filaments. This effectively corrupted the dynamic filament assemblies that link basal-cell nuclei to the extracellular matrix via α₆β₄-integrin and laminin-5. At 1 h postexposure, there was disruption in the surface organization of α₆β₄ integrins, associated displacement of laminin-5 anchoring sites and a concomitant loss of functional asymmetry. Accordingly, our multiphoton images are providing compelling evidence that SM induces prevesicating lesions that disrupt the receptor-ligand organization and cytoskeletal systems required for maintaining dermal-epidermal attachment, signal transduction, and polarized mobility.

Citation Download Citation

Robert J. S. Werrlein and Janna S. Madren-Whalley "Multiphoton imaging: a view to understanding sulfur mustard lesions", Proc. SPIE 4963, Multiphoton Microscopy in the Biomedical Sciences III, (10 July 2003); https://doi.org/10.1117/12.478035

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