Raman microscopy of cells: chemical imaging of apoptosis

Natallia Uzunbajakava; Jan Greve; Cees Otto

doi:10.1117/12.487609

10 July 2003 Raman microscopy of cells: chemical imaging of apoptosis

Natallia Uzunbajakava, Jan Greve, Cees Otto

Proceedings Volume 4963, Multiphoton Microscopy in the Biomedical Sciences III; (2003) https://doi.org/10.1117/12.487609
Event: Biomedical Optics, 2003, San Jose, CA, United States

Abstract

Singular value decomposition was applied to the set of the non-resonant Raman spectra, recorded during Raman imaging of the single apoptotic cell. The basis vectors and the corresponding singular values were assessed in terms of their statistical significance. The noise-containing basis vectors were rejected while keeping the meaningful ones. In this way the Raman images of the apoptotic cell were successfully reconstructed from the spectrally-filtered data matrix. The results are demonstrated on the spatial distribution of the DNA, protein and phospholipids, present in the fragments of the apoptotic cell.

Citation Download Citation

Natallia Uzunbajakava, Jan Greve, and Cees Otto "Raman microscopy of cells: chemical imaging of apoptosis", Proc. SPIE 4963, Multiphoton Microscopy in the Biomedical Sciences III, (10 July 2003); https://doi.org/10.1117/12.487609

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