Utilization of two-photon FRET to monitor SREBP homodimer and heterodimer formation in living cells

Vickie J. LaMorte; Aikaterini Zoumi; Shrimati Datta; Cristen J. Wu; Timothy Osborne

doi:10.1117/12.478034

10 July 2003 Utilization of two-photon FRET to monitor SREBP homodimer and heterodimer formation in living cells

Vickie J. LaMorte, Aikaterini Zoumi, Shrimati Datta, Cristen J. Wu, Timothy Osborne

Author Affiliations +

Proceedings Volume 4963, Multiphoton Microscopy in the Biomedical Sciences III; (2003) https://doi.org/10.1117/12.478034
Event: Biomedical Optics, 2003, San Jose, CA, United States

Abstract

Key players in cholesterol regulation are the members of a family of transcription factors known as the Sterol Regulatory Binding Proteins or SREBPs. The cellular redundancy of these proteins is under investigation, and our findings suggest that where these proteins reside may provide evidence for differences in the molecular dynamics of their transcriptional activity. Specifically, we have found that GFP-tagged SREBP-2 in contrast to SREBP-1 resides in discrete nuclear foci. To further explore functional differences between SREBP-1 and SREBP-2 we have developed an approach to monitor hetero- and homodimer formation by two-photon imaging and spectroscopy of fluorescence resonance energy transfer (TPIS-FRET). TPIS-FRET results will be presented. Collectively, these findings support the possibility that differences in function between SREBP family members may be governed by their localization within the cell.

Citation Download Citation

Vickie J. LaMorte, Aikaterini Zoumi, Shrimati Datta, Cristen J. Wu, and Timothy Osborne "Utilization of two-photon FRET to monitor SREBP homodimer and heterodimer formation in living cells", Proc. SPIE 4963, Multiphoton Microscopy in the Biomedical Sciences III, (10 July 2003); https://doi.org/10.1117/12.478034

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KEYWORDS

Fluorescence resonance energy transfer

Luminescence

Proteins

Imaging spectroscopy

Two photon imaging

Green fluorescent protein

In vivo imaging

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