Two-photon fluorescence microscopy imaging of biotissue: a theoretical model

Ekaterina A. Sergeeva

doi:10.1117/12.518624

13 October 2003 Two-photon fluorescence microscopy imaging of biotissue: a theoretical model

Ekaterina A. Sergeeva

Proceedings Volume 5068, Saratov Fall Meeting 2002: Optical Technologies in Biophysics and Medicine IV; (2003) https://doi.org/10.1117/12.518624
Event: Saratov Fall Meeting 2002 Laser Physics and Photonics, Spectroscopy, and Molecular Modeling III; Coherent Optics of Ordered and Random Media III, 2002, Saratov, Russian Federation

Abstract

We propose a theoretical modeling of a turbid medium images formed by two-photon fluorescent microscopy technique. On the basis of this model, general properties of the calculated images, such as the registered fluorescence intensity dependence on the focusing depth and the influence of the scattering in the medium, have been studied. A comparison of image properties for single-photon and two-photon microscopy is given.

Citation Download Citation

Ekaterina A. Sergeeva "Two-photon fluorescence microscopy imaging of biotissue: a theoretical model", Proc. SPIE 5068, Saratov Fall Meeting 2002: Optical Technologies in Biophysics and Medicine IV, (13 October 2003); https://doi.org/10.1117/12.518624

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