Local amplitude and phase retrieval method for digital holography applied to microscopy

Michael Liebling; Thierry Blu; Etienne Cuche; Pierre Marquet; Christian D. Depeursinge; Michael Unser

doi:10.1117/12.500517

14 October 2003 Local amplitude and phase retrieval method for digital holography applied to microscopy

Michael Liebling, Thierry Blu, Etienne Cuche, Pierre Marquet, Christian D. Depeursinge, Michael Unser

Proceedings Volume 5143, Novel Optical Instrumentation for Biomedical Applications; (2003) https://doi.org/10.1117/12.500517
Event: European Conferences on Biomedical Optics 2003, 2003, Munich, Germany

Abstract

We present a numerical two-step reconstruction procedure for digital off-axis Fresnel holograms. First, we retrieve the amplitude and phase of the object wave in the CCD plane. For each point we solve a weighted linear set of equations in the least-squares sense. The algorithm has O(N) complexity and gives great flexibility. Second, we numerically propagate the obtained wave to achieve proper focus. We apply the method to microscopy and demonstrate its suitability for the real time imaging of biological samples.

Citation Download Citation

Michael Liebling, Thierry Blu, Etienne Cuche, Pierre Marquet, Christian D. Depeursinge, and Michael Unser "Local amplitude and phase retrieval method for digital holography applied to microscopy", Proc. SPIE 5143, Novel Optical Instrumentation for Biomedical Applications, (14 October 2003); https://doi.org/10.1117/12.500517

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