LEDs for fluorescence microscopy

Ian T. Young; Yuval Garini; Heidi R. C. Dietrich; Wim van Oel; Guus Liqui Lung

doi:10.1117/12.525932

13 July 2004 LEDs for fluorescence microscopy

Ian T. Young, Yuval Garini, Heidi R. C. Dietrich, Wim van Oel, Guus Liqui Lung

Proceedings Volume 5324, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XI; (2004) https://doi.org/10.1117/12.525932
Event: Biomedical Optics 2004, 2004, San Jose, CA, United States

Abstract

Traditional light sources for fluorescence microscopy have been mercury lamps, xenon lamps, and lasers. These sources have been essential in the development of fluorescence microscopy but each can have serious disadvantages: lack of near monochromaticity, heat generation, cost, lifetime of the light source, and possible distortions due to coherence effects. We are examining the possibility of using the new high-power LED light sources as alternatives to the above mentioned sources. LED sources are near monochromatic, are inexpensive, produce little heat, have no coherence problems, have extended lifetimes, are small, and can easily be modulated. In this presentation we will describe experiments comparing various LEDs to other light sources. We will compare, for example, a 530 nm LED to the 546 nm line from a mercury lamp on a fluorophore whose absorption maximum is broad and in the middle between these two wavelengths.

Citation Download Citation

Ian T. Young, Yuval Garini, Heidi R. C. Dietrich, Wim van Oel, and Guus Liqui Lung "LEDs for fluorescence microscopy", Proc. SPIE 5324, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XI, (13 July 2004); https://doi.org/10.1117/12.525932

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