The point-spread function of fluorescence microscope imaging through an inhomogeneous medium

Olivier Haeberle; Bertrand Simon

doi:10.1117/12.545792

10 September 2004 The point spread function of fluorescence microscopes imaging through a layered or an inhomogeneous medium

Olivier Haeberle, Bertrand Simon

Author Affiliations +

Proceedings Volume 5462, Biophotonics Micro- and Nano-Imaging; (2004) https://doi.org/10.1117/12.545792
Event: Photonics Europe, 2004, Strasbourg, France

Abstract

The optical microscope has proven to be an invaluable tool in biology, because of its unique capabilities of 3-D imaging of living specimens. However, compared to other techniques, the achievable resolution is limited. Several techniques have been proposed to improve the resolution of the fluorescence microscope. The confocal set-up is the first of them. Interference effects can also be used to sharpen up the point spread function (PSF) in 4Pi microscopy. Another approach is the so-called STimulated Emission Depletion microscopy, which has permitted to decrease the resolution down to about 100 nm in three dimensions, and below 50 nm in either the x-y plane, or along the z-axis.

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Olivier Haeberle and Bertrand Simon "The point spread function of fluorescence microscopes imaging through a layered or an inhomogeneous medium", Proc. SPIE 5462, Biophotonics Micro- and Nano-Imaging, (10 September 2004); https://doi.org/10.1117/12.545792

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