Structure of insoluble immune complexes as studied by spectroturbidimetry and dynamic light scattering

Boris N. Khlebtsov; Gennadii L. Burygin; Larisa Yu. Matora; Sergei Yu. Shchyogolev; Nikolai G. Khlebtsov

doi:10.1117/12.568567

14 July 2004 Structure of insoluble immune complexes as studied by spectroturbidimetry and dynamic light scattering

Boris N. Khlebtsov, Gennadii L. Burygin, Larisa Yu. Matora, Sergei Yu. Shchyogolev, Nikolai G. Khlebtsov

Proceedings Volume 5475, Saratov Fall Meeting 2003: Coherent Optics of Ordered and Random Media IV; (2004) https://doi.org/10.1117/12.568567
Event: Saratov Fall Meeting 2003, 2003, Saratov, Russian Federation

Abstract

We describe two variants of a method for determining the average composition of insoluble immune complex particles (IICP). The first variant is based on measuring the specific turbidity (the turbidity per unit mass concentration of the dispersed substance) and the average size of IICP determined from dynamic light scattering (DLS). In the second variant, the wavelength exponent (i.e., the slope of the logarithmic turbidity spectrum) is used in combination with specific turbidity measurements. Both variants allow the average biopolymer volume fraction to be determined in terms of the average refractive index of IICP. The method is exemplified by two experimental antigen+antibody systems: (i) lipopolysaccharide-protein complex (LPPC) of Azospirillum brasilense Sp245+rabbit anti-LPPC; and (ii) human IgG (hIgG)+sheep anti-hIgG. Our measurements by the two methods for both types of systems gave, on the average, the same result: the volume fraction of the IICP biopolymers is about 30%; accordingly, the volume fraction of buffer solvent is 70%.

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Boris N. Khlebtsov, Gennadii L. Burygin, Larisa Yu. Matora, Sergei Yu. Shchyogolev, and Nikolai G. Khlebtsov "Structure of insoluble immune complexes as studied by spectroturbidimetry and dynamic light scattering", Proc. SPIE 5475, Saratov Fall Meeting 2003: Coherent Optics of Ordered and Random Media IV, (14 July 2004); https://doi.org/10.1117/12.568567

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