Digital analysis of fluorescent images as a method of assessing the advancement of cancerous disease

Grzegorz Tyc; Zbigniew Palasz; Elzbieta M. Beres-Pawlik; Tomasz Krecicki

doi:10.1117/12.581840

8 September 2004 Digital analysis of fluorescent images as a method of assessing the advancement of cancerous disease

Grzegorz Tyc, Zbigniew Palasz, Elzbieta M. Beres-Pawlik, Tomasz Krecicki

Proceedings Volume 5576, Lightguides and their Applications II; (2004) https://doi.org/10.1117/12.581840
Event: Lightguides and their Applications II, 2003, Krasnobrod, Poland

Abstract

Investigations of autofluorescence of cancerous tissues are used in clinical diagnostics. They are only estimative and allow us to define with close approximation the area of cancerously changed tissue. The paper presents a digital analysis of autofluorescent images. The analysis was conducted in the Matlab software environment. Pictures in the RGB range of colors were processed, obtained in similar lighting conditions exciting autofluorescence. As the result of digital processing distributions of intensity were obtained for three basic colors (red, green and blue). The intensity distributions of green and red were shown in three-dimensional space, where the points of the tissue examined were identified on the x y plane, and the intensity bound up with a given color was depicted in the third dimension. On the basis of results obtained this way the intensity ratio of green to red was defined in a selected spot in the examined tissue, and on this basis an attempt at assessing the advancement of cancer was made.

Citation Download Citation

Grzegorz Tyc, Zbigniew Palasz, Elzbieta M. Beres-Pawlik, and Tomasz Krecicki "Digital analysis of fluorescent images as a method of assessing the advancement of cancerous disease", Proc. SPIE 5576, Lightguides and their Applications II, (8 September 2004); https://doi.org/10.1117/12.581840

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