Determination of multiple components in urine using FT-MIR, NIR, and FT-Raman spectroscopic technique

Weiling Liu; Kexin Xu; Qilian Yu; Sixiang Zhang; Duogang Ran

doi:10.1117/12.571816

10 January 2005 Determination of multiple components in urine using FT-MIR, NIR, and FT-Raman spectroscopic technique

Weiling Liu, Kexin Xu, Qilian Yu, Sixiang Zhang, Duogang Ran

Proceedings Volume 5640, Infrared Components and Their Applications; (2005) https://doi.org/10.1117/12.571816
Event: Photonics Asia, 2004, Beijing, China

Abstract

This paper is centered on the development of reagent-free method based on Fourier transform mid infrared (FT-MIR), near infrared red (NIR) and Fourier transform (FT)-Raman spectroscopy. Suitable spectral wavenumber regions were selected for principal least square (PLS) regression and calibration models developed. In MIR, urine sample measured with Horizontal ATR, which is the accessory of Perkin-Elmer Spectrum GX Spectrometer, and the important range is 900-1800 cm^-1. NIR spectra are obtained for each sample with 1mm pathlength. Absorption spectra of urine samples in 5000~4200cm^-1 is used for PLS regression. The Raman spectra were recorded with a Bruker IFS 66 spectrometer with an FRA 106 FT-Raman accessory unit. The FT-Raman spectra of albumin power and albumin solution is recorded. The main noise is form water. The root mean square error of prediction (RMSEP) of glucose is 34.9 mg/dL(MIR), 31.56mg/dL(NIR) , and the RMSEP of albumin is 23.2mg/dL (MIR), 17.9mg/dL(NIR). Result indicated that FT-MIR and NIR spectroscopy could be used for rapid analysis of urine. FT-Raman spectroscopy could applied for compositional identification.

Citation Download Citation

Weiling Liu, Kexin Xu, Qilian Yu, Sixiang Zhang, and Duogang Ran "Determination of multiple components in urine using FT-MIR, NIR, and FT-Raman spectroscopic technique", Proc. SPIE 5640, Infrared Components and Their Applications, (10 January 2005); https://doi.org/10.1117/12.571816

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