Femtosecond laser application in biotechnology and medicine

Karsten Koenig

doi:10.1117/12.596315

8 October 2004 Femtosecond laser application in biotechnology and medicine

Karsten Koenig

Proceedings Volume 5662, Fifth International Symposium on Laser Precision Microfabrication; (2004) https://doi.org/10.1117/12.596315
Event: Fifth International Symposium on Laser Precision Microfabrication, 2004, Nara, Japan

Abstract

Near-infrared (NIR) 80 MHz nanojoule femtosecond laser pulses of low sub-nanojoule and nJ pulse energies in combination with focusing optics of high numerical aperture can be used as versatile multiphoton tools in nanobiotechnology and nano/micro-medicine. Novel diagnostic applications include gene imaging by multiphoton multicolor FISH (MM-FISH) and high-resolution multiphoton tomography of skin as well as tissue engineered cardiovascular structures based on two-photon autofluorescence excitation and second harmonic generation (SHG) of endogenous biomolecules. Using high-intense (10¹¹ - 10¹² W/cm²) 80 MHz femtosecond laser beams, non-invasive targeted transfection of mammalian cells with DNA can be realized by creation of highly localized membrane perforations. Nanosurgery can be performed by optical knocking out of intracellular and intratissue structures. Potential applications include gene and cancer therapy, eye and brain surgery as well as optical engineering of single DNA molecules as key elements in bionanotechnology.

Citation Download Citation

Karsten Koenig "Femtosecond laser application in biotechnology and medicine", Proc. SPIE 5662, Fifth International Symposium on Laser Precision Microfabrication, (8 October 2004); https://doi.org/10.1117/12.596315

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available