A novel PDMS microfluidic spotter for fabrication of protein chips and microarrays

David A. Chang-Yen; David Myszka; Bruce K. Gale

doi:10.1117/12.592151

22 January 2005 A novel PDMS microfluidic spotter for fabrication of protein chips and microarrays

David A. Chang-Yen, David Myszka, Bruce K. Gale

Proceedings Volume 5718, Microfluidics, BioMEMS, and Medical Microsystems III; (2005) https://doi.org/10.1117/12.592151
Event: MOEMS-MEMS Micro and Nanofabrication, 2005, San Jose, California, United States

Abstract

A novel PDMS microfluidic spotter system has been developed for the patterning of surface microarrays that require individually addressing each spot area and high probe density. Microfluidic channels are used to address each spot region and large spot arrays can be addressed in parallel. Fluorescence intensity measurement of dye-spotted samples compared to control and pipetted drops demonstrated a minimum of a three-fold increase in dye surface density. Surface plasmon resonance measurement of protein-spotted samples as compared to pin-spotted samples demonstrated an 86-fold increase in protein surface density. This novel spotter system can be applied to the production of high-throughput arrays in the fields of genomics, proteomics, immunoassays and fluorescence or luminescence assays.

Citation Download Citation

David A. Chang-Yen, David Myszka, and Bruce K. Gale "A novel PDMS microfluidic spotter for fabrication of protein chips and microarrays", Proc. SPIE 5718, Microfluidics, BioMEMS, and Medical Microsystems III, (22 January 2005); https://doi.org/10.1117/12.592151

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available