Miniature fluorescence detection system for protein chips

Hoseong Kim; Jaeho Choi; Kook-Nyung Lee; Yongkwon Kim

doi:10.1117/12.583281

22 January 2005 Miniature fluorescence detection system for protein chips

Hoseong Kim, Jaeho Choi, Kook-Nyung Lee, Yongkwon Kim

Proceedings Volume 5718, Microfluidics, BioMEMS, and Medical Microsystems III; (2005) https://doi.org/10.1117/12.583281
Event: MOEMS-MEMS Micro and Nanofabrication, 2005, San Jose, California, United States

Abstract

We report the development of miniature fluorescence detection systems that employ miniature prism, mirrors and low cost CCD camera to detect the fluorescence emitted from 40 fluorescently-labeled protein patterns without scanner. This kind of miniature fluorescence detection systems can be used in point of care. We introduce two systems, one uses prism + mirror block and the other uses prism and two mirrors. A large NA microscope eyepiece and low cost CCD camera are used. We fabricated protein chip containing multi-pattern BSA labeled with Cy5, using MEMS technology and modified the surface chemically to clean and to immobilize proteins. The measurements show that the combination of prism and mirrors can homogenize elliptical excitation light over the sample with higher optical efficiency, and increase the separation between excitation and fluorescence light at the CCD to give higher signal intensity and higher signal to noise ratio. The measurements also show that protein concentrations ranging from 10 ng/ml to 1000 ng/ml can be assayed with very small error. We believe that the proposed fluorescence detection system can be refined to build a commercially valuable hand-held or miniature detection device.

Citation Download Citation

Hoseong Kim, Jaeho Choi, Kook-Nyung Lee, and Yongkwon Kim "Miniature fluorescence detection system for protein chips", Proc. SPIE 5718, Microfluidics, BioMEMS, and Medical Microsystems III, (22 January 2005); https://doi.org/10.1117/12.583281

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KEYWORDS

Fluorescence spectroscopy

Luminescence

Prisms

Proteins

Mirrors

Glasses

CCD cameras

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