Time- and wavelength-resolved autofluorescence detection by multi-dimensional TCSPC

Wolfgang Becker; Axel Bergmann; Christoph Biskup; Dietrich Schweitzer; Martin Hammer

doi:10.1117/12.633033

7 October 2005 Time- and wavelength-resolved autofluorescence detection by multi-dimensional TCSPC

Wolfgang Becker, Axel Bergmann, Christoph Biskup, Dietrich Schweitzer, Martin Hammer

Proceedings Volume 5862, Diagnostic Optical Spectroscopy in Biomedicine III; 58620S (2005) https://doi.org/10.1117/12.633033
Event: European Conferences on Biomedical Optics 2005, 2005, Munich, Germany

Abstract

We present a multi-dimensional TCSPC technique that simultaneously records the photon distribution over the time in the fluorescence decay, the wavelength, and the coordinates of a two-dimensional scan. We demonstrate the application of the technique to single-point autofluorescence measurements of skin, to multi-spectral fluorescence lifetime microscopy, and ophthalmic imaging.

Citation Download Citation

Wolfgang Becker, Axel Bergmann, Christoph Biskup, Dietrich Schweitzer, and Martin Hammer "Time- and wavelength-resolved autofluorescence detection by multi-dimensional TCSPC", Proc. SPIE 5862, Diagnostic Optical Spectroscopy in Biomedicine III, 58620S (7 October 2005); https://doi.org/10.1117/12.633033

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