Gold nanoparticles probe for recognition of specific DNA sequence based on non-crosslink system

Zheng Zhou; Da Xing; Debin Zhu

doi:10.1117/12.710947

27 October 2006 Gold nanoparticles probe for recognition of specific DNA sequence based on non-cross-link system

Zheng Zhou, Da Xing, Debin Zhu

Proceedings Volume 6047, Fourth International Conference on Photonics and Imaging in Biology and Medicine; 60473X (2006) https://doi.org/10.1117/12.710947
Event: Fourth International Conference on Photonics and Imaging in Biology and Medicine, 2005, Tianjin, China

Abstract

Simpler, cheaper method for DNA sequence recognition is of a greater scientific and economic interest. In this paper, we develop a novel colorimetric detection method for recognition of specific DNA. The detection method bases on the strong distance-dependent optical properties of gold nanoparticles (Au-nps) and the DNA sequences' electrostatic interactions with the Au-nps. As a result of the single-stranded DNA (ssDNA) displaying far higher affinities than double-stranded DNA (dsDNA) to negatively charged Au-nps, when the concentration of salt is added, Au-nps in ssDNA keep stable because of the absorption of large amount of ssDNA, the mixture's color is red. But Au-nps in dsDNA aggregate immediately, and the mixture's color turns out to be blue. Therefore, we can judge whether the hybridization occurs by the mixture's color or absorption spectrums. Au-nps aggregation in this detection mode is not induced by hybridization of target DNA which cross-links the nanoparticles but by salt, then the color transition is rapid and clearly visible. This method is simple, cheap, and we can detect about 2Opmol specific DNA sequences.

Citation Download Citation

Zheng Zhou, Da Xing, and Debin Zhu "Gold nanoparticles probe for recognition of specific DNA sequence based on non-cross-link system", Proc. SPIE 6047, Fourth International Conference on Photonics and Imaging in Biology and Medicine, 60473X (27 October 2006); https://doi.org/10.1117/12.710947

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KEYWORDS

Gold

Absorption

Nanoparticles

Optical properties

Target detection

Photography

Visible radiation

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