Detection apparatus for fluorescent microarray slides

Liqiang Wang; Zukang Lu

doi:10.1117/12.674232

9 June 2006 Detection apparatus for fluorescent microarray slides

Liqiang Wang, Zukang Lu

Proceedings Volume 6149, 2nd International Symposium on Advanced Optical Manufacturing and Testing Technologies: Advanced Optical Manufacturing Technologies; 614918 (2006) https://doi.org/10.1117/12.674232
Event: 2nd International Symposium on Advanced Optical Manufacturing and Testing Technologies, 2005, Xian, China

Abstract

Detection of fluorescent microarray slides can be divided into two categories: one is confocal scanning detection in which a photomultiplier tube is used as fluorescence sensor, and the other is flood illumination of the entire microarray slides and uses a Peltier cooled CCD as fluorescence sensor. CCD can afford high quantum efficiency, simultaneous illumination and detection of multiple pixels, and easy mechanical design, but its dynamic range and sensitivity are not as good as those of photomultiplier tube. At the same time, the Peltier cooled CCD camera is much more expensive than a high performance photomultiplier tube. Confocal scanning provides high dynamic range, good sensitivity and high signal-to-noise ratio, but the system design is difficult when considering rapid scanning speed, high resolution and large numerical aperture. There are three typical confocal scanning apparatuses which are mechanical scanning, optical scanning and optical-mechanical scanning. Their scanning mechanism, advantage and disadvantage are analyzed respectively. With the former understanding, a new optical-mechanical scanning apparatus is described in detail. It employs two lasers to excite the Cy3 and Cy5 fluorophores on the microarray slides. The emitted fluorescent signal is detected using a photomultiplier tube sequentially. One dimension scanning of the slides is performed by a telecentric f-θ objective with a moving coil optical scanner; the other dimension is scanned through a stepping motor driving a precision guidance. This apparatus is low-noise, economical and fast in scanning speed.

Citation Download Citation

Liqiang Wang and Zukang Lu "Detection apparatus for fluorescent microarray slides", Proc. SPIE 6149, 2nd International Symposium on Advanced Optical Manufacturing and Testing Technologies: Advanced Optical Manufacturing Technologies, 614918 (9 June 2006); https://doi.org/10.1117/12.674232

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
6 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Objectives

Confocal microscopy

Optical scanning

Scanners

Sensors

Photomultipliers

Luminescence

Show All Keywords

Keywords/Phrases

Search In:

Publication Years