Gene transfer of human hepatocyte growth factor by the use of nanosecond pulsed laser-induced stress waves

Mitsuhiro Terakawa; Shunichi Sato; Daizoh Saitoh; Makoto Hasegawa; Hiroshi Ashida; Hideyuki Okano; Minoru Obara

doi:10.1117/12.668965

7 June 2006 Gene transfer of human hepatocyte growth factor by the use of nanosecond pulsed laser-induced stress waves

Mitsuhiro Terakawa, Shunichi Sato, Daizoh Saitoh, Makoto Hasegawa, Hiroshi Ashida, Hideyuki Okano, Minoru Obara

Proceedings Volume 6261, High-Power Laser Ablation VI; 626133 (2006) https://doi.org/10.1117/12.668965
Event: High-Power Laser Ablation 2006, 2006, Taos, NM, United States

Abstract

We successfully delivered a therapeutic vector construct, which carries hepatocyte growth factor (HGF) gene, to rat skin in vivo. After HGF expression vector had been intradermally injected to rat skin, LISWs were generated by irradiating the laser target put on the rat skin with nanosecond pulses from the second harmonics (532 nm) of a Q-switched Nd:YAG laser. Concentration of HGF protein increased by a factor of four by the application of LISWs when compared with that of control samples without LISW application. We also investigated the effects of LISWs on the integrity of plasmid DNA.

Citation Download Citation

Mitsuhiro Terakawa, Shunichi Sato, Daizoh Saitoh, Makoto Hasegawa, Hiroshi Ashida, Hideyuki Okano, and Minoru Obara "Gene transfer of human hepatocyte growth factor by the use of nanosecond pulsed laser-induced stress waves", Proc. SPIE 6261, High-Power Laser Ablation VI, 626133 (7 June 2006); https://doi.org/10.1117/12.668965

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
4 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Skin

Proteins

In vivo imaging

Pulsed laser operation

Q switched lasers

Laser therapeutics

Defense and security

Show All Keywords

Keywords/Phrases

Search In:

Publication Years