Encapsulation of cell into monodispersed hydrogels on microfluidic device

Chang-Hyoung Choi; Ji-Hye Lee; Hyun-Woo Shim; Nae-Rym Lee; Jae-Hoon Jung; Tae-Ho Yoon; Dong-Pyo Kim; Chang-Soo Lee

doi:10.1117/12.708548

14 December 2006 Encapsulation of cell into monodispersed hydrogels on microfluidic device

Chang-Hyoung Choi, Ji-Hye Lee, Hyun-Woo Shim, Nae-Rym Lee, Jae-Hoon Jung, Tae-Ho Yoon, Dong-Pyo Kim, Chang-Soo Lee

Proceedings Volume 6416, Biomedical Applications of Micro- and Nanoengineering III; 641613 (2006) https://doi.org/10.1117/12.708548
Event: SPIE Smart Materials, Nano- and Micro-Smart Systems, 2006, Adelaide, Australia

Abstract

In here, we present the microfluidic approach to produce monodispersed microbeads that will contain viable cells. The utilization of microfludics is helpful to synthesize monodispersed alginate hydrogels and in situ encapsulate cell into the generating hydrogels in microfludic device. First, the condition of formation of hydrogels in multiphase flows including oil, CaCl₂, and alginate was optimized. Based on the preliminary survey, microfludic device could easily manipulate the size of alginate beads having narrow size distribution. The microfluidic method manipulates the size of hydrogel microbeads from 30 to 200um with a variation less than 2%. For the proof of concept of cell entrapment, the live yeast expressing green fluorescence protein is successfully encapsulated in microfluidic device.

Citation Download Citation

Chang-Hyoung Choi, Ji-Hye Lee, Hyun-Woo Shim, Nae-Rym Lee, Jae-Hoon Jung, Tae-Ho Yoon, Dong-Pyo Kim, and Chang-Soo Lee "Encapsulation of cell into monodispersed hydrogels on microfluidic device", Proc. SPIE 6416, Biomedical Applications of Micro- and Nanoengineering III, 641613 (14 December 2006); https://doi.org/10.1117/12.708548

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available