Multimodal confocal mosaicing of basal cell carcinomas in Mohs surgical skin excisions

Daniel S. Gareau; Yogesh G. Patel; Yongbiao Li; Kishwer S. Nehal; Billy Huang; Milind Rajadhyaksha

doi:10.1117/12.700672

14 February 2007 Multimodal confocal mosaicing of basal cell carcinomas in Mohs surgical skin excisions

Daniel S. Gareau, Yogesh G. Patel, Yongbiao Li, Kishwer S. Nehal, Billy Huang, Milind Rajadhyaksha

Proceedings Volume 6431, Multimodal Biomedical Imaging II; 64310U (2007) https://doi.org/10.1117/12.700672
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

Mohs surgery is a procedure for microscopically excising basal cell carcinomas (BCCs) while preserving maximal surrounding normal skin. Each serial excision is guided by examination of the frozen histology of the previous excision. Because several (2-20) excisions must be made and frozen histology prepared for each excision. Mohs surgery is time-consuming (15-45 minutes per excision) and tedious. Real-time confocal reflectance mosaicing enables detection of BCCs directly in fresh excisions, following contrast-enhancement by acetowhitening. A confocal mosaic allows rapid observation of 15x15 mm2 of tissue, which is equivalent to a low magnification, 2X view of the excision. Relatively large superficial nodular and micronodular BCCs are rapidly detectable in confocal reflectance mosaics, whereas detection of much smaller infiltrative and sclerosing BCCs is a challenge due to the lack of sufficient nuclear/dermis contrast in acetowhitened excisions. Multimodal contrast, combining reflectance with either fluorescence or autofluorescence may make it possible to detect infiltrative and sclerosing BCCs. A reflectance image shows both nuclei and the surrounding dermis, whereas an autofluorescence image (excitation at 488nm, detection 500-700nm) shows only the dermis. Thus, ability of a composite (i.e., reflectance-less-autofluorescence) image shows significantly darkened dermis, with stronger enhancement of nuclear/dermis contrast. Preliminary results illustrate that this may enable detection of infiltrative and sclerosing BCCs. The use of reflectance and autofluorescence parallels the use of two stains (hematoxylin and eosin) in histology, thus allowing a more complete optical detection method.

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Daniel S. Gareau, Yogesh G. Patel, Yongbiao Li, Kishwer S. Nehal, Billy Huang, and Milind Rajadhyaksha "Multimodal confocal mosaicing of basal cell carcinomas in Mohs surgical skin excisions", Proc. SPIE 6431, Multimodal Biomedical Imaging II, 64310U (14 February 2007); https://doi.org/10.1117/12.700672

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