Numerical optix: a time-domain simulator of fluorescent-light diffusion in turbid medium

Guobin Ma; Jean-François Delorme; Olga Guilman; Frédéric Leblond; Mario Khayat

doi:10.1117/12.701251

13 February 2007 Numerical optix: A time-domain simulator of fluorescent light diffusion in turbid medium

Guobin Ma, Jean-François Delorme, Olga Guilman, Frédéric Leblond, Mario Khayat

Proceedings Volume 6434, Optical Tomography and Spectroscopy of Tissue VII; 64342J (2007) https://doi.org/10.1117/12.701251
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

The interest in fluorescence imaging has increased steadily in the last decade. Using fluorescence techniques, it is feasible to visualize and quantify the function of genes and the expression of enzymes and proteins deep inside tissues. When applied to small animal research, optical imaging based on fluorescent marker probes can provide valuable information on the specificity and efficacy of drugs at reduced cost and with greater efficiency. Meanwhile, fluorescence techniques represent an important class of optical methods being applied to in vitro and in vivo biomedical diagnostics, towards noninvasive clinical applications, such as detecting and monitoring specific pathological and physiological processes. ART has developed a time domain in vivo small animal fluorescence imaging system, eXplore Optix. Using the measured time-resolved fluorescence signal, fluorophore location and concentration can be quickly estimated. Furthermore, the 3D distribution of fluorophore can be obtained by fluorescent diffusion tomography. To accurately analyze and interpret the measured fluorescent signals from tissue, complex theoretical models and algorithms are employed. We present here a numerical simulator of eXplore Optix. It generates virtual data under well-controlled conditions that enable us to test, verify, and improve our models and algorithms piecewise separately. The theoretical frame of the simulator is an analytical solution of the fluorescence diffusion equation. Compared to existing models, the coupling of fluorophores with finite volume size is taken into consideration. Also, the influences of fluorescent inclusions to excitation and emission light are both accounted for. The output results are compared to Monte-Carlo simulations.

Citation Download Citation

Guobin Ma, Jean-François Delorme, Olga Guilman, Frédéric Leblond, and Mario Khayat "Numerical optix: A time-domain simulator of fluorescent light diffusion in turbid medium", Proc. SPIE 6434, Optical Tomography and Spectroscopy of Tissue VII, 64342J (13 February 2007); https://doi.org/10.1117/12.701251

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KEYWORDS

Luminescence

Monte Carlo methods

Diffusion

Tissues

Sensors

Computer simulations

Reflectivity

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