Photodynamic therapy induced production of cytokines by latent Epstein Barr virus infected epithelial tumor cells

H. K. Koon; K. W. Lo; M. L. Lung; C. K. C. Chang; R. N. S. Wong; N. K. Mak

doi:10.1117/12.710533

13 February 2007 Photodynamic therapy induced production of cytokines by latent Epstein Barr virus infected epithelial tumor cells

H. K. Koon, K. W. Lo, M. L. Lung, C. K. C. Chang, R. N. S. Wong, N. K. Mak

Author Affiliations +

Proceedings Volume 6438, Biophotonics and Immune Responses II; 64380M (2007) https://doi.org/10.1117/12.710533
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

Photodynamic therapy (PDT) is a method to treat cancer or non-cancer diseases by activation of the light-sensitive photosensitizers. Epstein Barr virus (EBV) has been implicated in the development of certain cancers such as nasopharyngeal carcinoma and B cell lymphoma. This study aims to examine the effects of EBV infection on the production of pro-inflammatory cytokines and chemokines in cells after the photosensitizer Zn-BC-AM PDT treatment. Epithelial tumor cell lines HONE-1 and latent EBV-infected HONE-1 (EBV-HONE-1) cells were used in this study. Cells were treated with the photosensitizer Zn-BC-AM for 24 hours before light irradiation. RT-PCR and quantitative ELISA methods were used for the evaluation of mRNA expression and production of cytokines, respectively. Results show that Zn-BC-AM PDT increases the production of IL-1a and IL-1b in EBV-HONE-1. Over a 10-fold increase in the production of IL-6 was observed in the culture supernatant of Zn-BC-AM PDT-treated HONE-1 cells. PDT-induced IL-6 production was observed in HONE-1 cells. EBV-HONE-1 has a higher background level of IL-8 production than the HONE-1. The production of IL-8 was suppressed in EBV-HONE-1cells after Zn-BC-AM PDT. Our results indicate that the response of HONE-1 cells to Zn-BC-AM PDT depends on the presence of latent EBV infection. Since IL-8 is a cytokine with angiogenic activity, Zn-BC-AM PDT may exert an anti-angiogenic effect through the suppression of IL-8 production by the EBV-infected cells.

Citation Download Citation

H. K. Koon, K. W. Lo, M. L. Lung, C. K. C. Chang, R. N. S. Wong, and N. K. Mak "Photodynamic therapy induced production of cytokines by latent Epstein Barr virus infected epithelial tumor cells", Proc. SPIE 6438, Biophotonics and Immune Responses II, 64380M (13 February 2007); https://doi.org/10.1117/12.710533

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