Enhancement of spontaneous burst activity of hippocampal neuronal networks with low frequency close-loop stimulation

Yanling Li; Wei Zhou; Shaoqun Zeng; Xiangning Li; Man Liu; Qingming Luo

doi:10.1117/12.741465

1 May 2007 Enhancement of spontaneous burst activity of hippocampal neuronal networks with low frequency close-loop stimulation

Yanling Li, Wei Zhou, Shaoqun Zeng, Xiangning Li, Man Liu, Qingming Luo

Author Affiliations +

Proceedings Volume 6534, Fifth International Conference on Photonics and Imaging in Biology and Medicine; 65343C (2007) https://doi.org/10.1117/12.741465
Event: Fifth International Conference on Photonics and Imaging in Biology and Medicine, 2006, Wuhan, China

Abstract

In this paper, we study the modulation of low frequency closed-loop electric stimulation on spontaneous activity in cultured hippocampal neuronal networks. First, we plated monolayer cultures of hippocampal neurons from rat embryos (E18) on multi-electrode arrays and the experiments were performed in the networks from the second week to the sixth week continuously. During the experiments, we detected the spontaneous spikes of the networks firstly, and then stimulated the networks at low frequency (0.2 Hz or 1 Hz) stimulation respectively until a desired response was observed 20-80msec after a stimulus. The protocol was closed-loop. After that, we detected the spontaneous spikes of the networks. It is observed that the spontaneous activity in the developing networks is developing, which is oscillatory and periodic. Low frequency (0.2 Hz or 1 Hz) stimulation enhanced the spontaneous synchronous burst activity of the developing networks. These results implicated that activity-dependent mechanism in the modulation of plasticity of synaptic transmission in the cultured neuronal networks. Closed-loop stimulation will give a better view on the functional significance of networks activities. Besides, close-loop stimulation could set up the stimulus-reward system in the neuronal networks, which is of great benefit to the plasticity of synaptic transmission in the cultured neuronal networks.

Citation Download Citation

Yanling Li, Wei Zhou, Shaoqun Zeng, Xiangning Li, Man Liu, and Qingming Luo "Enhancement of spontaneous burst activity of hippocampal neuronal networks with low frequency close-loop stimulation", Proc. SPIE 6534, Fifth International Conference on Photonics and Imaging in Biology and Medicine, 65343C (1 May 2007); https://doi.org/10.1117/12.741465

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
10 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Neurons

Electrodes

Action potentials

In vitro testing

Modulation

Synaptic transmission

Plating

Show All Keywords

Keywords/Phrases

Search In:

Publication Years