Frequency domain fluorescence diffuse tomography of small animals

Anna G. Orlova; Ilya V. Turchin; Vladislav A. Kamensky; Vladimir I. Plehanov; Irina V. Balalaeva; Ekaterina A. Sergeeva; Marina V. Shirmanova; Michail S. Kleshnin

doi:10.1117/12.741469

1 May 2007 Frequency domain fluorescence diffuse tomography of small animals

Anna G. Orlova, Ilya V. Turchin, Vladislav A. Kamensky, Vladimir I. Plehanov, Irina V. Balalaeva, Ekaterina A. Sergeeva, Marina V. Shirmanova, Michail S. Kleshnin

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Proceedings Volume 6534, Fifth International Conference on Photonics and Imaging in Biology and Medicine; 65343G (2007) https://doi.org/10.1117/12.741469
Event: Fifth International Conference on Photonics and Imaging in Biology and Medicine, 2006, Wuhan, China

Abstract

Fluorescent compounds for selective cancer cell marking are used for development of novel medical diagnostic methods, investigation of the influence of external factors on tumor growth, regress and metastasis. Only special tools for turbid media imaging, such as optical diffusion tomography permit noninvasive monitoring of fluorescent-labeled tumor alterations deep in animal tissue. In this work, the results of preliminary experiments utilizing frequency-domain fluorescent diffusion tomography (FD FDT) experimental setup in small animal are presented. Low-frequency modulated light (1 kHz) from Nd:YAG laser with second harmonic generation at the wavelength of 532 nm was used in the setup. The transilluminative planar configuration was used in the setup. A series of model experiments has been conducted and show good agreement between theoretical and experimental fluorescence intensity. Models of deep tumors were created by two methods: (1) glass capsules containing fluorophore solution were inserted into esophagus of small animals to simulate marked tumors; (2) a suspension of transfected HE&Kgr;293-Turbo-RFP cells was subcutaneously injected to small animal. The conducted experiments have shown that FD FDT allows one to detect the presence of labeled tumor cells in small animals, to determine the volume of an experimental tumor, to perform 3D tumor reconstruction, as well as to conduct monitoring investigations. The obtained results demonstrate the potential capability of the FD FDT method for noninvasive whole-body imaging in cancer studies, diagnostics and therapy.

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Anna G. Orlova, Ilya V. Turchin, Vladislav A. Kamensky, Vladimir I. Plehanov, Irina V. Balalaeva, Ekaterina A. Sergeeva, Marina V. Shirmanova, and Michail S. Kleshnin "Frequency domain fluorescence diffuse tomography of small animals", Proc. SPIE 6534, Fifth International Conference on Photonics and Imaging in Biology and Medicine, 65343G (1 May 2007); https://doi.org/10.1117/12.741469

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KEYWORDS

Luminescence

Tumors

Animal model studies

Scattering

Tomography

Sensors

Glasses

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