Multifocal multiphoton microscopy using a novel field of view zoom scanning protocol

Lixin Liu; Lei Wang; Junle Qu; Ziyang Lin; Zhe Fu; Wenquing Liu; Hanben Niu

doi:10.1117/12.727965

11 July 2007 Multifocal multiphoton microscopy using a novel field of view zoom scanning protocol

Lixin Liu, Lei Wang, Junle Qu, Ziyang Lin, Zhe Fu, Wenquing Liu, Hanben Niu

Proceedings Volume 6628, Diagnostic Optical Spectroscopy in Biomedicine IV; 662814 (2007) https://doi.org/10.1117/12.727965
Event: European Conferences on Biomedical Optics, 2007, Munich, Germany

Abstract

We present a novel field of view (FOV) zoom scanning multifocal multiphoton microscopy (MMM) system that is based on a microlens array for producing discrete excitation spot array, and the combining of a pair of galvo mirrors and a sample stage for changing the resolution and FOV with fixed optical system and objective. The system can be operated not only in low resolution and large FOV, but also in high resolution and small FOV applications, without compromising the performances of the optical elements in the system. By implementing dedicated system control protocol and image reconstruction algorithm, fluorescence images in different FOV and resolution can be obtained according to user's definition. The performance of the system is demonstrated with two-photon excitation fluorescence imaging of a fluorescence resolution test target, prepared plant stem slide and fluorescence microspheres suspension. Preliminary results indicate that the system has high spatial resolution and holds potential for providing high image acquisition rate. This FOV zoom scanning protocol can be used in our simultaneous time- and spectrum-resolved MMM system, which can provide spectral and lifetime information simultaneously in fluorescence microscopy for biomedical imaging.

Citation Download Citation

Lixin Liu, Lei Wang, Junle Qu, Ziyang Lin, Zhe Fu, Wenquing Liu, and Hanben Niu "Multifocal multiphoton microscopy using a novel field of view zoom scanning protocol", Proc. SPIE 6628, Diagnostic Optical Spectroscopy in Biomedicine IV, 662814 (11 July 2007); https://doi.org/10.1117/12.727965

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KEYWORDS

Luminescence

Zoom lenses

Image resolution

Imaging systems

Mirrors

Multiphoton microscopy

Optical scanning systems

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