A laser-induced fluorescence optical fiber detection system for micro-fluidic chip

Hong Wang; Yu Tang

doi:10.1117/12.769425

25 January 2008 A laser-induced fluorescence optical fiber detection system for micro-fluidic chip

Hong Wang, Yu Tang

Proceedings Volume 6836, MEMS/MOEMS Technologies and Applications III; 68360O (2008) https://doi.org/10.1117/12.769425
Event: Photonics Asia 2007, 2007, Beijing, China

Abstract

Based on optic fiber beam sensor, a miniaturized laser induced fluorescence optical fiber detection system using solid-state laser (473nm) as excitation source is developed. The advantage of the LIF-D system are simple structure, small size, low cost, high sensitivity and high anti-disturbing. And the photon count method is used to improve the sensitivity of the detection. Using Sodium fluorescein as the test sample, distinct fluorescence signal with good repeatability is showed in the experiment. The concentration detection limit is up to 3×10^-13mol/L. the linear dynamic range is from 10^-6 to 10^-13 mol/L. It also shown that there was a nicer linear relation between the peak value of fluorescence signal to Sodium fluorescein concentration. Its relation coefficient is 0.99954. By changing detection cells this integrated detection system can be applied in micro flow analysis and for the normal biotechnology analysis of DNA.

Citation Download Citation

Hong Wang and Yu Tang "A laser-induced fluorescence optical fiber detection system for micro-fluidic chip", Proc. SPIE 6836, MEMS/MOEMS Technologies and Applications III, 68360O (25 January 2008); https://doi.org/10.1117/12.769425

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